![]() ![]() Based on the assignment of the 13C resonances, the 1H chemical shifts are derived in a straightforward manner using one-bond 1H– 13C correlations from heteronuclear experiments (HC–CT–HSQC). The 13C resonances are traced using 13C– 13C correlations from homonuclear experiments, such as (H)CC–CT–COSY, (H)CC–NOESY, CC–CT–TOCSY and/or virtually decoupled (H)CC–TOCSY. The first step in this approach involves the assignment of the carbon resonances in each monosaccharide spin system using the anomeric carbon signal as the starting point. Two model substances were employed: one compound of low molecular weight and one compound of medium molecular weight ( 13C-enriched O-antigenic polysaccharide isolated from Escherichia coli O142, ~10 kDa). In this study, a set of nuclear magnetic resonance experiments, some of them commonly used in the study of 13C-labeled proteins and/or nucleic acids, is applied for the structure determination of uniformly 13C-enriched carbohydrates.
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